Journal of Food, Agriculture and Environment




Vol 7, Issue 3&4,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Antimicrobial activities of protenic extracts of Allium roseum L., a wild edible species in North Africa


Author(s):

Hanen Najjaa 1*, Emna Ammar 2, Mohamed Neffati 1

Recieved Date: 2009-06-12, Accepted Date: 2009-09-22

Abstract:

This work was carried out on Allium roseum L., a North African endemic species, growing in the South-East of Tunisia and used as a vegetable, spice or herbal remedy in traditional medicine. Three protocols of extraction (Tris HCl buffer, phosphate buffer and sulphuric acid) were used in order to extract proteins from four different organs of this species (flowers, bulbs, leaves and seeds). Tris HCl buffer extract showed the highest content of A. roseum protein. Leaves had the highest protein content and seed had the highest diversified molecular weight varying from 10 to 50 kDa comparatively to the other organs. Antimicrobial activities of different protein extracts were tested by disc diffusion and well diffusion and evaluated by the determination of minimum inhibitory concentration (CMI) and minimum bactericidal concentration (MBC). Antimicrobial activities of the extracts against ten tested microbial strains differed depending on the extraction method and plant material used. The determination of CMI and CMB relieved a very significant antimicrobial activity to some strains such as C. albicans and E. coli. Extracts selected for highest antimicrobial activity were tested for sensitivity to heat and proteases. Tested extracts were thermolabile and their activities were sensitive to several proteases including α-chymotrypsine, pepsin and trypsin. Results suggest the potential use of the plant as condiment and preservative in the food industry.

Keywords:

Tunisia, antimicrobial activity, peptides, food pathogens


Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 3&4
Category: Food and Health
Pages: 150-154


View full PDF
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :