Journal of Food, Agriculture and Environment
Vol 9,
Issue 3&4,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255
|
|
|
|
Newsletter
Subscribe to our newsletter and stay up to date with the latest publishes
Development of an enzyme-linked immunosorbent assay for detection of ofloxacin residue in environment matrix
Author(s):
Xiaolin Hou 1,
Kaijun Guo 1, Jie Bao 2, Yongnin Wu 3, Huanrong Li 1*Recieved Date: 2011-06-19, Accepted Date: 2011-09-30
Abstract:Ofloxacin polyclonal antibody was prepared and cross-reactivity to other fluroquinones was observed. Further, a rapid, low-cost ELISA analytical technique was developed, with IC50 at 1.2 ng.mL-1, for the analysis of ofloxacin in the environment matrix including wastewater, manure and sludge. The mean recoveries and coefficients of variation were in a range of 74-83% and 8.5-12.2%, respectively, in wastewater. The mean recoveries and coefficients of variation in pig manure and sludge were from 62% to 78% and from 8.4% to 14.4%, respectively. Concentrations of ofloxacin using ELISA were sometimes greater than measured with LC-MS/MS. It may be due to cross-reactivity to structurally similar compounds or metabolites. Results from this study showed that the ELISA can be used as a preliminary screening tool for later instrumental analysis.
Keywords:Ofloxacin,
ELISA, wastewater, manure, sludgeJournal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 3&4
Category: Environment
Pages: 779-783
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :