Journal of Food, Agriculture and Environment

QTL analysis of mercury tolerance and accumulation at the seedling stage in rice (Oryza sativa L.)

Author(s):

You Jian Yu, Hai Tao Hu, Chang Chun Wang, Ling Yang ^*

Recieved Date: 2011-01-07, Accepted Date: 2011-04-04

Abstract:

Mercury (Hg) is one of the most toxic heavy metals to living organisms and its conspicuous effect was inhibition of seedling growth. Twenty rice genotypes were grown under different Hg conditions to investigate the relative biomass of the root, shoot and entire plant, and the results indicated that different genotypes showed different relative biomass under different Hg conditions. Two Hg-tolerant (IR64 and 9311) and one Hg-sensitive (Azucena) rice varieties were screened. To investigate the genetic basis of Hg tolerance in rice at the seedling stage in nutrient solutions with or without 80 µM of HgCl₂, a population of 96 doubled haploid lines derived from the cross between a Hg-tolerant indica variety IR64 and a Hg-sensitive japonica variety Azucena with 284 molecular markers was used to identify the quantitative trait loci (QTLs). A total of seven QTLs for the relative parameters of the shoot height (SH), root biomass (RB), shoot biomass (SB) and total biomass (TB) were detected on chromosomes 1, 3, 6 and 8 with LOD score ranging from 2.70 to 9.45 and the phenotypic variance explained from 6.94 to 36.17%. The positive contributions of five QTLs were from the tolerant parent IR64. A major QTL on chromosome 1 between R210 and AMY1B for relative total biomass (RTB) could explain 36% of the phenotypic variation. The Hg-tolerant variety IR64 had higher Hg concentration (p<0.01) in both shoot and root than the sensitive variety Azucena. Three QTLs for Hg accumulation including two for Hg concentration in leaf (HgL) and one for Hg concentration in root (HgR) were also determined. Two of three accumulation alleles were contributed by the Hg-tolerant parent IR64. These results provide an insight into the genetic basis of Hg tolerance and accumulation in rice and will be useful in identifying genes associated with Hg tolerance and accumulation.

Keywords:

Oryza sativa L., mercury stress, tolerance, accumulation, QTLs

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Environment
Pages: 748-752

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