Journal of Food, Agriculture and Environment

Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)

Author(s):

Fatimah Abdul Rahiman ^*, Rosna Mat Taha

Recieved Date: 2011-01-12, Accepted Date: 2011-04-16

Abstract:

Tissue culture studies of Lawsonia inermis, syn. Lawsonia alba (henna) were carried out to induce multiple shoots, plant regeneration and coloured callus on MS media supplemented with various hormones. Various explants, such as sections of leaves, stems and roots, from 4-month-old aseptic seedlings were used. Different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), napthalene acetic acid (NAA), benzyl aminopurine (BAP), kinetin, and indole acetic acid (IAA) hormones were used. The results showed that efficient regeneration system for henna plant in vitro could be achieved. Different coloured callus were obtained from henna tissue culture on MS supplemented with different combinations and concentrations of hormones 0.5 mg/L 2,4-D and 0.5 mg/L IAA, 2.0 mg/L NAA and 2.0 mg/L BAP, 0.5 mg/L NAA and 0.5 mg/L BAP, 1.5 mg/L NAA and 1.5 mg/L BAP, and also 0.5 mg/L IAA and 0.5 mg/L BAP, and thus production of important secondary metabolites in vitro is possible for this important species. Formation of embryogenic callus was observed during the plant morphogenesis in MS medium supplemented with combination of 0.5 mg/L IAA and 0.5 mg/L 2,4-D hormones which will enhance more production of in vitro plantlets and ultimately promote new propagules of this species.

Keywords:

Tissue culture, Murashige and Skoog (MS), multiple shoot, aseptic seedlings, 2,4-dichlorophenoxyacetic acid (2,4-D), napthalene acetic acid (NAA), benzyl aminopurine (BAP), indole acetic acid (IAA), in vitro, embryogenic callus

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 397-399

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