Journal of Food, Agriculture and Environment

Studies on determining the appropriate hormone concentrations on meristem culture of some strawberry (Fragaria spp.) cultivars

Author(s):

Nafiye Adak

Recieved Date: 2011-01-10, Accepted Date: 2011-04-03

Abstract:

Present work was conducted to reveal the propagation possibilities of Camarosa, Chandler and Oso Grande strawberry cultivars in meristem culture by using different doses and combinations of BAP(benzylaminopurine), IAA (indoleacetic acid) and GA₃ (gibberellic acid) as well as active charcoal (AC) in rooting stage. In the meristem growth and development stage, 1.0 mg L^-1 BAP and 1.0 mg L^-1 IAA in the propagation stage, different concentrations and combinations of BAP, IAA and GA₃were evaluated. In the propagation stage a) 0, 0.5, 1.0, 1.5 and 2.0 mg L^-1 BAP + 1.0 mg L^-1 IAA + 1.0 mg L^-1 GA₃, b) 1.0 mg L^-1 BAP + 1.0 mg L^-1 IAA + 0, 0.1, 0.4, 0.8 and 1.0 mg L^-1 GA₃ and c) 1.0 mg L^-1 BAP + 0, 0.1, 0.4, 0.8 and 1.0 mg L^-1 IAA + 1.0 mg L^-1 GA₃ were studied. In case of the rooting stage different doses of AC (0, 2.0, 4.0 and 6.0 g L^-1) were also investigated. As a result, the doses to be recommended were found as follows: 1.0 mg L^-1 BAP for each; 0.4, 0.8 and 1.0 mg L^-1 GA₃ for Camarosa; 0.1 mg L^-1 GA₃ for Chandler and 0.4 mg L^-1 GA₃ for Oso Grande. Regarding IAA, 1.0 mg L^-1 IAA was found to be the most appropriate concentration for the number of shoots and quality. In case of rooting stage, elevated doses of AC engendered an increase in the number of roots, the length of the root, number of leaves and stem diameter in each strawberry cultivar.

Keywords:

Strawberry, cultivars, tissue culture, propagation, rooting

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 341-344

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