Journal of Food, Agriculture and Environment
Vol 9,
Issue 2,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Molecular characterization and preliminary functional analysis of cystatin OC-I in rice
Author(s):
Shijuan Dou 1*,
Jian Wei 1, Yinghao Cao 2, Jimping Lan 1Recieved Date: 2011-01-17, Accepted Date: 2011-04-10
Abstract:There are 12 cystatin genes in rice, whose gene structures and chromosomal location were determined based on the genomic sequences of rice (Oryza sativa L.). However, the expression pattern and biological functions of the corresponding genes still remain unclear. In this study, a gene, OC-I encoding a cysteine proteinase inhibitor was experimentally characterized, which can efficiently inhibit papain (cysteine proteinase). The MPSS and EST analysis showed that OC-I is expressed in roots, leaves and panicles. Notably, OC-I can be transphosphorylated by XA21K in vitro. Accordingly, the OC-I gene may probably participate in the disease-resistance pathway mediated by bacterial blight resistance gene Xa21 in rice.
Keywords:Cystatin,
OC-I, rice, Xa21, phosphorylationJournal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 235-239
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