Journal of Food, Agriculture and Environment

Vol 9, Issue 2,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Evaluation of genetic divergence among traditional accessions of common bean by using RAPD molecular markers


Marco Antonio A. Barelli 1, Juliana P. Poletine 2*, Cláudia Thomazella 3, Pedro S. Vidigal Filho 2, Elieser Souto 2, Cinthya M. N. A. Pacheco 2, Leonarda Grillo Neves 1, Julio César Ferreira Elias 1, Maria Celeste Gonçalves-Vidigal 2

Recieved Date: 2011-01-18, Accepted Date: 2011-03-29


The objective of this study was to evaluate genetic divergence among 30 traditional cultivars of common bean from Mato Grosso do Sul State (Brazil) by using RAPD molecular markers. Genetic diversity among accessions was evaluated by DNA electrophoretic banding patterns; presence or absence of bands (coded 1 or 0, respectively) provided data for constructing a similarity matrix based on Jaccard’s coefficient. This matrix was converted to an inverse dissimilarity matrix, allowing a cluster analysis (using the hierarchical nearest neighbor procedure) of traditional accessions. RAPD analyses produced 97 DNA bands from the 30 accessions, and cluster analysis demonstrated the presence of four distinct groups. The first group was subdivided into three classes, of which only one was of Andean origin. Mulatinho Vagem Roxa B and Jalo accessions were the most divergent (91.80%), while Rosinha B and Rosinha Opaco were the most similar (18.67%). The precise genetic divergence pattern that was uncovered allowed to distinguish Andean and Mesoamerican genotypes. Therefore, the best strategy to be adopted is the use of the combination Mulatinho Vagem Roxa B x Jalo in interpopulational breeding program in order to obtain superior segregants.


Traditional cultivars, Phaseolus vulgaris L., genetic diversity, molecular markers

Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 195-199

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