Journal of Food, Agriculture and Environment

A modified method for isolation and culture of adipocyte precursors from pig adipose tissue

Author(s):

Zuo-Hua Liu ^1#, Jin-Long Yang ^1#, Fei-Yun Yang ^1#, Ding-Biao Long ^1#, Kang-Cheng Pan ^2*, Dai-Wen Chen ^3*

Recieved Date: 2010-10-21, Accepted Date: 2011-01-09

Abstract:

The paper details a modified method for isolation and culture of adipocyte precursors from pig adipose tissue. We modified the ceiling culture method for pig mature adipocytes, which use their buoyant property to allow them better adhere to a floating glass surface, where they remain viable for several weeks. The optimized conditions for adipose more cell culture is the collagenase concentration of 1mg/ml, digestion duration of two hours and FBS concentration of 15%. The method which overcomes the shortcomings of adipocyte suspension culture, can be used to study adipocyte cell biology.

Keywords:

Adipocyte precursors, culture

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Food and Health
Pages: 45-46

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