Journal of Food, Agriculture and Environment
Vol 7,
Issue 2,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255
|
|
|
|
Newsletter
Subscribe to our newsletter and stay up to date with the latest publishes
Initiation of in vitro cultures of Lycopersicon peruvianum var. humifusum
Author(s):
D. Rzepka-Plevnes *,
D. Kulpa, A. WajdaRecieved Date: 2009-01-22, Accepted Date: 2009-04-18
Abstract:The aim of this research was to determine the initiation capacity of wild tomato L. peruvianum var. humifusum. Two kinds of in vitro culture were induced – direct shoot regeneration on explants (apical meristem, axillary bud, leaf base) and indirect via callus. In the first variant 12 concentration combinations of IBA or NAA or KIN or BAP were used and in the second one – 13 combinations of auxin NAA with cytokinin BAP. It was found that clonal micropropagation of L. peruvianum var. humifusum is possible on MS medium with 3 or 4 mg·dm-3 IBA whereas embryogenic callus induction on MS medium supplemented with 0.5 mg·dm-3 NAA and 5 mg·dm-3 BAP. After two months 34 embryos and 1 regenerated plant were obtained from 2.205g of embryogenic callus. Clonal micropropagation, in the majority of cases did not produce genetically homogenous clones.
Keywords:Lycopersicum var. humifusum,
clonal micropropagation, inter simple sequence repeats, polymorphismJournal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Agriculture
Pages: 576-580
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :