Journal of Food, Agriculture and Environment

mRNA cnf1 detection for monitoring viability and pathogenicity in an uropathogenic Escherichia coliJ96 maintained in laboratory seawater microcosm

Author(s):

Denden Ikbel ^*, Mahdouani Kassem, Ben Chibani Jemni, Bakhrouf Amina

Recieved Date: 2004-01-18, Accepted Date: 2004-04-22

Abstract:

The seawater effects on persistence of necrotizing Escherichia coli (NTEC) pathogenicity were studied using an uropathogenic strain,Escherichia coli J96, previously incubated for 24 hours in waste water at stationary phase of growth. Using PCRessais, we have found that E.coli J96 strains exposed for long periods in seawater retained cnf1 gene. This gene was also expressed during the viable state in laboratory microcosms through detection of E.coli cnf1 mRNA by reverse transcription-PCR over a 5-month period. Maintenance of E.coli J96 pathogenicity was shown even when the culture returned to active growth in restored optimal conditions. The presence of cnf1 mRNA at viable state (culturable and VBNC) and in resuscitated cells correlating with cytotoxic activity on HEp2 cells and necrotic activity in rabbit skin, indicated the viability and the maintenance of pathogenicity during E.coli J96 incubation in seawater microcosms.

Keywords:

Escherichia coli, cnf1 mRNA, viability, expression of cnf1 gene, seawater stress, pathogenicity

---

Journal: Journal of Food, Agriculture and Environment
Year: 2004
Volume: 2
Issue: 2
Category: Food and Health
Pages: 100-103

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :