Journal of Food, Agriculture and Environment

Detection of Tomato spotted wilt virus in North-East of Iran

Author(s):

Alireza Mohammadi Hajiabadi ¹, Behrooz Jafarpour ¹, Mahrokh Falahat Rastegar ¹, Babak Abdollahi Mandoulakani ^2*

Recieved Date: 2009-01-09, Accepted Date: 2009-04-25

Abstract:

In 2002 and 2007, Tomato spotted wilt virus (TSWV) was detected in tomato leaf and fruit samples collected from Khorasane-razavi (Mashhad, Chenaran, Quchan, Torbate-hedarieh and Neishabour regions) and North-khorasan (Bojnourd and Shirvan regions) provinces in North-East of Iran. Presence of TSWV was tested by bioassay and also DAS-ELISA. Bioassay was performed using indicator test plants including Petunia hybrida, Vigna unguiculata, Nicotiana glutinosa and N. rustica. Small brownish local necrotic lesions appeared on P. hybrida and V. unguiculata. Systemic symptoms on N. glutinosa and N. rustica included concentric ring spots on leaves, stem necrosis, wilting and tissue collapse. Tomato (Lycopersicon esculentum) necrotic brown spots appeared on the leaves after the inoculation, stem necrosis and yellow concentric ring spots on reddish fruits also appeared. In 2002, among 630 plant samples collected from various regions in Khorasane-razavi and North Khorasan provinces, just 12 samples out of 300 tomato samples were infected with TSWV (Torbate-hedarieh, Neishabour and Shirvan regions). In 2007, among 700 plant samples collected from mentioned regions, only 25 samples out of 320 tomato samples showed positive reactions for TSWV in ELISA test and the remaining samples were negative (Mashhad, Torbate-hedarieh, Neishabour, Bojnourd and Shirvan regions). Antiserum prepared against a partially purified TSWV preparation cross-reacted with tomato samples.

Keywords:

Tomato spotted wilt virus, DAS-ELISA, indicator test plants

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Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Agriculture
Pages: 471-474

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