Journal of Food, Agriculture and Environment

Vol 7, Issue 2,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Detection of Tomato spotted wilt virus in North-East of Iran


Alireza Mohammadi Hajiabadi 1, Behrooz Jafarpour 1, Mahrokh Falahat Rastegar 1, Babak Abdollahi Mandoulakani 2*

Recieved Date: 2009-01-09, Accepted Date: 2009-04-25


In 2002 and 2007, Tomato spotted wilt virus (TSWV) was detected in tomato leaf and fruit samples collected from Khorasane-razavi (Mashhad, Chenaran, Quchan, Torbate-hedarieh and Neishabour regions) and North-khorasan (Bojnourd and Shirvan regions) provinces in North-East of Iran. Presence of TSWV was tested by bioassay and also DAS-ELISA. Bioassay was performed using indicator test plants including Petunia hybridaVigna unguiculataNicotiana glutinosa and N. rustica. Small brownish local necrotic lesions appeared on P. hybrida and V. unguiculata. Systemic symptoms on N. glutinosa and N. rustica included concentric ring spots on leaves, stem necrosis, wilting and tissue collapse. Tomato (Lycopersicon esculentum) necrotic brown spots appeared on the leaves after the inoculation, stem necrosis and yellow concentric ring spots on reddish fruits also appeared. In 2002, among 630 plant samples collected from various regions in Khorasane-razavi and North Khorasan provinces, just 12 samples out of 300 tomato samples were infected with TSWV (Torbate-hedarieh, Neishabour and Shirvan regions). In 2007, among 700 plant samples collected from mentioned regions, only 25 samples out of 320 tomato samples showed positive reactions for TSWV in ELISA test and the remaining samples were negative (Mashhad, Torbate-hedarieh, Neishabour, Bojnourd and Shirvan regions). Antiserum prepared against a partially purified TSWV preparation cross-reacted with tomato samples.


Tomato spotted wilt virus, DAS-ELISA, indicator test plants

Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Agriculture
Pages: 471-474

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :