Journal of Food, Agriculture and Environment
Vol 7,
Issue 2,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Callus formation and shoot organogenesis in Moshgak (Ducrosia flabellifolia Boiss.) from cotyledon
Author(s):
Manoochehr Karimi 1,
Amin Ebrahimi 1, Amir Sahraroo 2*, Seyed-Amir Moosavi 1, Firoozeh Moosavi 1, Mohammad-Reza Bihamta 1Recieved Date: 2009-01-12, Accepted Date: 2009-04-16
Abstract:An in vitro protocol has been developed for plant regeneration through callus induction in the medicinal plant Moshgak, Ducrosia flabellifolia Boiss. (Apiaceae), using cotyledons as explants. Modified B5 medium supplemented with NAA and BA was used. Different concentration of D-sorbitol was also examined in shooting and rooting phases. Results showed that B5 medium plus 7.5 mg/l NAA and 4 mg/l BA was the best medium for callus induction. The highest shoot initiation was observed in modified B5 medium containing 1.5 mg/l BA and 3% D-sorbitol. Rooting of microshoots was achieved in the basal medium supplemented with 0.5 mg/l NAA. In this phase, D-sorbitol had no significant effect on root number but it significantly reduced fresh weight of plantlets (p<0.01). After acclimatization the survival plantlets were transferred to the greenhouse conditions. This optimized protocol is suitable for vegetative propagation and secondary metabolism studies.
Keywords:Apiaceae,
basal medium, callus induction, D-sorbitol, Ducrosia flabellifolia BoissJournal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Agriculture
Pages: 441-445
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