Journal of Food, Agriculture and Environment

Vol 7, Issue 2,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Chemical composition, antibacterial and antioxidant activities of a new chemotype of Tunisian Thymus vulgaris oils growing in Sayada


Belsem Marzouk 1, Hayet Edziri 1, Ihsen Haloui 2, Manel Issawi 3, Imed Chraief 3, Mahjoub El-Ouni 1, Nadia Fnina 2, Zohra Marzouk 4*

Recieved Date: 2009-01-21, Accepted Date: 2009-04-15


Tunisia recently increased research in traditional herbal medicines after observation that they are effective for conditions to which they have traditionally applied. Present study is a scientific approach to re-establish the traditional uses of Tunisian Thymus vulgaris, to describe the chemical composition of its essential oils and to evaluate their antibacterial and antioxidant activities. The essential oils, obtained from fresh and dried leaves and flowers (in bud and in bloom) by hydrodistillation, were analysed by gas chromatography and mass spectrometry. The antimicrobial activity was tested against four reference strains by the dilution method and the antioxidant effect was evaluated. Thirty eight compounds were identified. In all oils, oxygen containing monoterpenes were the most abundant chemical group (from 80.9% in fresh flowers in bud to 88.8% in dried flowers in bloom), especially carvacrol (75.73% and 84.81% respectively). The oils displayed an antimicrobial activity. Escherichia coli and Enterococcus faecalis are the most sensitive, particularly to oils from fresh flowers in bud with the same MIC value of 0.2% and MBC values of 1.6 and 0.4%, respectively. All the extracts decreased significantly the signal of DPPH radicals (up to 98.43%).


Thymus vulgaris L., essential oils, chemical composition, antibacterial activity, antioxidant activity, Sayada

Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Food and Health
Pages: 263-267

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