Journal of Food, Agriculture and Environment
Characterization and bioactivity of water-soluble polysaccharides from the fruit of pumpkin
Author(s):
Junli Li 2,
Yunqiang Wang 1, 3, De Zhang 1, Xiaohua Hu 4, Zhengqin Zhang 4, Changping Xiang 1*Recieved Date: 2010-01-10, Accepted Date: 2010-04-18
Abstract:Four fractions of water-soluble polysaccharide, coded as PP1, PP2, PP3 and PP4, were isolated and purified from the fruit of pumpkin at 20°C, 40°C, 60°C and 80°C, respectively. Their chemical structure and physico-chemical properties were characterized by FT-IR spectra, gas chromatography and viscometry, respectively. Furthermore, their antioxidant activities were evaluated in vitro. The results indicated that four fractions of pumpkin polysaccharide were all composed of L-arabinose (Ara), L-rhamnose (Rha), D-glucose (Glu), D-galactose (Gal) and D-glucuronic acid, with variation of the component ratio. The polysaccharides showed polyelectrolyte effect due to the existing of glucuronic acid. PP1 showed the highest scavenging rate on hydroxyl radicals and superoxide radicals among four fractions of pumpkin polysaccharide. The four polysaccharide fractions were found to have the ability to alleviate the liver mitochondria swelling at concentration 2 mg/ml. The scavenging rate on lipid peroxidation in liver homogenate by anti-oxidation of PP1, PP2, PP3 and PP4 were 64.09%, 55.3%, 35.4% and 49.9% at concentration 2.5 mg/ml, respectively.
Keywords:Pumpkin polysaccharides,
characterization, antioxidant activitiesJournal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Food and Health
Pages: 237-241
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :