Journal of Food, Agriculture and Environment

Vol 7, Issue 2,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Ascorbic acid and flavanone glycosides in citrus: Relationship with antioxidant activity


Maria Graça Miguel *, Amílcar Duarte, Susana Nunes, Vera Sustelo, Denise Martins, Susana Anahi Dandlen

Recieved Date: 2009-01-14, Accepted Date: 2009-03-29


The effects of cultivar and orchard on the content of ascorbic acid and flavanone glycosides as well as on the antioxidant activity of citrus juices were studied. Ascorbic acid and flavanone glycosides were determined using HPLC method. For antiradicalar activity evaluation, TAEC and ORAC methods were used. Citrus of ‘Valencia late’ from Faro and ‘D. João’ presented the highest levels of hesperidin in contrast to the citrus of ‘Valencia late’ from Silves with 0.4 mg/ml, a value closer to those of Navel oranges ‘Navelate’ and ‘Lanelate’. Citrus of ‘Valencia late’ from Silves and ‘D. João’ presented the lowest levels of narirutin. ‘Ortanique’ and ‘Encore’ cultivars were poorer in ascorbic acid compared with the oranges. When the TEAC method was used for the determination of antioxidant activity, ‘Valencia late’ from Faro presented the best activity. A good relation was observed between antiradical activity measured through the TEAC method and the amount of ascorbic acids in samples. None relation was found between the levels of flavanones and antiradical activity. When the ORAC method was applied, the best activity was found for ‘Navelate’ and ‘Encore’ samples and practically none relation between this method and the amounts of ascorbic acid and flavanones was found.


Valencia late, Navelate, Lanelate, D. João, Encore, Ortanique, ORAC, TEAC, ascorbic acid, flavonoids

Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Food and Health
Pages: 222-227

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