Journal of Food, Agriculture and Environment

Assessment of genetic diversity in Trigonella foenum graecum Tunisian cultivars using ISSR markers

Author(s):

Nidhal Marzougui ^1*, Anissa Boubaya ¹, Walid Elfalleh ¹, Ferdaous Guasmi ¹, Leila Laaraiedh ¹, Ali Ferchichi ¹, Tebra Triki ¹, Mohamed Beji ²

Recieved Date: 2008-10-08, Accepted Date: 2008-12-11

Abstract:

Trigonella foenum graecum is an annual flowering species largely cultivated in the Mediterranean basin countries, central Asia, central Europe, United Kingdom and United States. The grain is rarely grown outside their native habitat. This work aims to study the diversity of 38 Tunisian cultivars of this plant using Inter Simple Sequence Repeat markers. Cultivars were collected from upper-semi arid, sub humid and lower arid Tunisian bioclimatic stages. A set of 12 ISSR primers was used for cultivars initial screening. Four primers gave amplification, detected intraspecific variation and revealed 144 bands. Sixty-two bands were polymorphic with an average of 15.5 bands per primer and 60.5% of polymorphism. The percentage of polymorphic bands varied from 30.7% for (CA)₆ GT primer to 73.3% for (GAG)₃GC primer. The highest heterozygosity value (0.95) was obtained with (GA)₆CC primer and the lowest one (0.5) was observed with (CA)₆GT primer. The highest marker index value (3.6) was recorded with (GAG)₃GC primer and the lowest one (2.25) was noted with (CA)₆AG. UPGMA dendrogram presented five cultivar clusters. Principal components analysis confirmed this clustering and showed the same five clusters that were obtained by UPGMA analysis. Genetic distances were not correlated with geographic distances (r = 0.04 and p = 0.366>0.05) but the majority of the cultivars were localized in the upper semi arid stage. This study confirmed the hypothesis of the Near East and Mediterranean Centers to be the centers of origin and/or diversity of Trigonella and suggested that Tunisia should be added to the list of countries belonging to the Mediterranean Center.

Keywords:

Trigonella foenum graecum, genetic diversity, bioclimatic stage, Tunisia, ISSR, polymorphism, heterozygosity, marker index, UPGMA, principal components analysis, center of origin and/or diversity

---

Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 1
Category: Agriculture
Pages: 101-105

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :