Journal of Food, Agriculture and Environment

Vol 2, Issue 1,2004
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Evaluation of Jordanian wheat and barley genotypes for anther culture response


Dhia S. Hassawi

Recieved Date: 2003-11-05, Accepted Date: 2004-01-29


The effects of genotypes and growth regulators on anther culture response were investigated using two wheat and five barley cultivars. Two separate experiments were conducted. In the first one, barley genotypes significantly affected anther response and callus production; callus induction frequency was (1.25%, 3.30%, 2.25%, 3.60% and 1.00 %) for cultivars (B1, Rum, B5, B8 and B9) respectively. In the second one, medium containing 2,4-D (2,4-dichlorophenoxyacetic acid) produced more calli and green plantlets than medium containing NAA (naphthalic acetic acid) from wheat cultivars (Acsad 65 and Hourani Nawawi). The wheat plantlets that reached maturity are fertile, healthy and could be useful as plant material for breeding program.


Wheat anther culture, barley anther culture, doubled haploid, callus

Journal: Journal of Food, Agriculture and Environment
Year: 2004
Volume: 2
Issue: 1
Category: Agriculture
Pages: 193-197

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