Journal of Food, Agriculture and Environment
Vol 5,
Issue 3&4,2007
Online ISSN: 1459-0263
Print ISSN: 1459-0255
|
|
|
|
Newsletter
Subscribe to our newsletter and stay up to date with the latest publishes
An efficient transformation system for sugarbeet mediated by Agrobacterium tumefaciens
Author(s):
Shawky A. Bekheet *,
Mohi E. SollimanRecieved Date: 2007-04-18, Accepted Date: 2007-08-20
Abstract:This paper describes the development of a reliable transformation protocol for sugarbeet which can be used for integration of foreign gene into plant genome. To optimize a transformation protocol for sugarbeet with Agrobacterium, bacterial strain LB4404 and a plasmid PBI121 containing kanamycin resistance and GUS genes were used in the transformation experiments. The influence of plant explants and amount of kanamycin added to selective medium were examined for their effect on transformation frequency. Among the different concentrations of kanamycin used, 100 mg/l was the optimum for both transformation frequency and survival of plant tissues. Leaf tissues showed high transformation frequency compared with shoot base explant. Transient expression of GUS in transformed cultures was assayed and large regions showed expression of GUS (blue patches). Polymerase Chain Reaction (PCR) was used to verify the presence of the transgenes in the selected cultures of sugarbeet. PCR analysis showed that high frequency of GUS positive cultures contained transgenes. The transformants were successfully developed to complete plants growing in free- living conditions.
Keywords:Sugarbeet,
transformation, Agrobacterium tumefaciensJournal: Journal of Food, Agriculture and Environment
Year: 2007
Volume: 5
Issue: 3&4
Category: Agriculture
Pages: 193-197
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :