Journal of Food, Agriculture and Environment

Vol 6, Issue 1,2008
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Preparation of cellooligosaccharides: Comparative study


Ozlem Akpinar 1*, Michael H. Penner 2

Recieved Date: 2007-10-07, Accepted Date: 2007-12-13


Cellooligosaccharides having a specific degree of polymerization are commonly used in mechanistic studies of cellulolytic enzymes and since they are not hydrolyzed or absorbed by the human gastrointestinal tract, (non-digestible oligosaccharides) they may have potential as non-caloric bulking agents in food products. Considering the heavy demand for cellooligosaccharides in many cellulase/cellulose laboratories, many methods were developed for the separation of cellooligosaccharides. They are typically produced by the controlled hydrolysis of cellulose, which produces a mixture of cellooligosaccharides differing with respect to their degree of polymerization, followed by chromatographic fractionation to obtain preparations homogeneous with respect to degree of polymerization. This research summarizes a comparative study of suggested fractionation methods. Charcoal-celite adsorption, molecular exclusion and ion-mediated silica and cellulose affinity/partition chromatography were evaluated. Adsorption chromatography with charcoal-celite stationary phases was the most generally applicable method for the preparation of near gram quantities of pure cellooligosaccharides in the DP range of three to seven. Cellulose affinity/partition chromatography was found to be the least time consuming and most economical method for the preparation of cellotetraose and cellopentaose. Ion-mediated chromatography was the most readily applicable for analytical applications.


Cellooligosaccharides, cellodextrin, cellulose, fractionation, prebiotics

Journal: Journal of Food, Agriculture and Environment
Year: 2008
Volume: 6
Issue: 1
Category: Food and Health
Pages: 55-61

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